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nanoscopy
Two-Dimensional Nanoscopy: Measuring Electron Oscillations
Researchers from Bielefeld, Kaiserslautern and Würzburg have developed a novel high-tech microscope: It magnifies objects a million times and shows movements with a retardation of one million billion times. The results were published in Science.The new technology allows tracking extremely fast processes in miniature objects - with an unparalleled spatial and temporal resolution. more
New Ways to Super-Resolution: Reversible Chemical Reactions Control Fluorescent States
Recently, light microscopy has been revolutionized by novel approaches that circumvent the diffraction barrier the resolution limit of optical microscopes. Most of these novel methods are based on light-controlled switching of the labels fluorescent states and therefore require use of additional or more intense laser excitation lines. To overcome these demands a new probe has been developed which is controlled by a reversible chemical reaction thereby reducing the demands on the microscopes.Limitations of Light Microscopy
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Live-Cell Super-Resolution with dSTORM
Live-Cell Super-Resolution with dSTORM: A detailed microscopic characterization of cellular structures is important to understand cellular function. Conventional microscopy in some cases is limited by the achievable spatial resolution of about 200 nm in the imaging plane, which is not sufficient to reveal details at the near-molecular level. This is important if the organization of proteins in small organelles, clusters or machineries are studied. more
Super Resolution Microscopy: Light-independent Switching of Fluorescent Probes
Super Resolution Microscopy: a team of scientists headed by Dr. Dirk-Peter Herten at Heidelberg University's Institute of Physical Chemistry and members of the Cluster of Excellence "CellNetworks" have devised a new method in which light-dependent processes are replaced by chemical reactions to mark cellular structures for high-resolution optical microscopy. This method opens up new application vistas for fluorescence microscopy. more
University of Manchester Scientists Breaking the Theoretical Limit of Optical Microscopes
University of Manchester research team, led by Professor Lin Li and Dr Zengbo Wang have created a microscope which shatters the record for the smallest object the eye can see, breaking the theoretical limit of optical microscopes. They wrote about their research in Nature Communications.Previously, the standard optical microscope can only see items around one micrometre - 0.001 millimetres - clearly. more
Nanoscope Testing at Marburg University in Cooperation with Leica Microsystems
The University of Marburg is expanding its cooperation with Leica Microsystems: The Institute of Cytobiology is currently one of four institutes in the world to test a microscope with a resolution well below the diffraction limit (nanoscope). "With this new optical nanoscopy called GSDIM (ground state depletion microscopy followed by individual molecule return), resolutions down to 25 nanometers can be achieved. more
The Fast Track to Superresolution
The Leica TCS STED CW is the most uncomplex way to nanoscopy for research. The system resolves structures smaller than 80 nm - with purely optical methods. The depletion lasers and emission lasers in the visible spectral range enable researchers to use conventional dyes such as Alexa 488, FITC and Oregon Green and beyond that established fluorescence proteins such as YFP. Dynamics of sub-cellular processes can be imaged due to this flexibility and the system's ability of fast data acquisition. This means: live cell imaging below the diffraction limit. moreDec. 01, 2008Photoswitchable Fluorescent Proteins for Nanoscopy
Photoswitchable Fluorescent Proteins for Nanoscopy. S. Jacobs and colleagues have recently presented several photoswitchable fluorescent proteins that are suitable for fluorescence nanoscopy techniques like photoactivation localisation microscopy (PALM). In the article by M. Andresen and co-workers (1), two different reversibly switchable fluorescent proteins bsDronpa and Padron with similar emissions but with opposing switching behaviours are described. A.C. more- Legal
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