optical trap
Dec. 29, 2009
The propulsion of the leading edge of neuronal lamellipodia is a complex process in which the polymerization of actin filaments towards the cell membrane is a major component [1,2]. This process is at the origin of force generation in neurons. By using optical tweezers, we have characterized the dynamics by which lamellipodia of Dorsal Root Ganglia neurons exerted force on encountered obstacles such as silica beads.
moreDec. 22, 2009
A prerequisite for an understanding of the mechanisms of nerve regeneration and neuronal development is the understanding of the principles of force generation in growth cones - the mechanical and chemical sensor of a neuron. In our working group we try to approach this issue from a variety of different aspects, combining experimental and theoretical studies of actin networks, membrane fluctuations and focal adhesion sites.
moreDec. 18, 2009
Optical trapping techniques have evolved to the point where quantitative force measurements on biological systems can be performed down into the femtonewton range. As resolution is constantly improving, the pinpointing and elimination of noise sources become increasingly important. Allan-variance analysis is ideally suited for this task; adjacent time series are recorded and the variations between observation intervals are calculated.
moreDec. 17, 2009
The powerful results in the molecular and cellular domain that are currently obtained using optical tweezers are leading to an increasing interest in this biophysical tool. Once calibrated, laser traps can be used to accurately measure the forces and displacements involved in many different molecular processes. Unfortunately, standard force detection techniques are not suitable for experiments inside living cells.
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