Apr. 10, 2012
Classical phase contrast microscopy techniques allow only non-quantitative partial visualization of transparent samples. In recent years, a variety of techniques have appeared for complete phase determination with different levels of complexity. A new simple non-interferometric method able to provide real time quantitative optical path difference information is presented. It is based on the use of a refractive pyramid to measure the transillumination wavefront gradient using incoherent light.
moreFeb. 01, 2006
Digital Holographic Microscopy (DHM): 3D Real-Time Optical Imaging at the Nanometer Scale. Digital Holographic Microscopes (DHM) enables strictly noninvasive visualisation of unstained transparent and partially reflective specimens, in real time, by providing simultaneously amplitude and phase changes of a light wave transmitted or reflected.
moreNov. 01, 2005
A Tool Kit for Imaging, Manipulating and Probing Biological Systems. The atomic force microscope (AFM) has become a powerful tool for studying various questions in biology. The mechanical nature of the microscope means that one instrument can be used to image, manipulate and measure forces. By then integrating the atomic force microscope into an inverted light microscope, multiple aspects of a system can be monitored, allowing physical interactions to be linked to structure and to function.