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Refractive Pyramide

Quantitative Phase Microscopy
Apr. 10, 2012

Quantitative Phase Microscopy

Classical phase contrast microscopy techniques allow only non-quantitative partial visualization of transparent samples. In recent years, a variety of techniques have appeared for complete phase determination with different levels of complexity. A new simple non-interferometric method able to provide real time quantitative optical path difference information is presented. It is based on the use of a refractive pyramid to measure the transillumination wavefront gradient using incoherent light.

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