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Structured Illumination Microscopy: Chemical Probe Profiles Live-cell Organelle Activity
Apr. 02, 2014

Structured Illumination Microscopy: Chemical Probe Profiles Live-cell Organelle Activity

A team of scientists from Pacific Northwest National Laboratory synthesized a chemical activity-based probe (ABP) that can provide new information about how living cells function. The new ABP is designed to enter a living cell without interacting with anything until it enters a specific organelle: the lysosome. This proof-of-concept ABP then labels only functionally active enzymes called cathepsins, which are cysteine proteases, in the lysosome. Using proteomics and super-resolution microscopy to view these labeled enzymes, the scientists now are able to see organellar activity. Their work, which demonstrates the ability to manipulate chemistry to better understand biology, has been published in Angewandte Chemie International Edition.
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Live Cell Super-Resolution Microscopy with Plastic Bottom Slides
Oct. 16, 2012

Live Cell Super-Resolution Microscopy with Plastic Bottom Slides

ibidi has announced the use of its ยต-Slides, which have a special plastic bottom, for super-resolution imaging applications based on the microscopy method dSTORM. more
BioEssays Special Issue on Light Microscopy: Free Access
Apr. 26, 2012

BioEssays Special Issue on Light Microscopy: Free Access

BioEssays special issue on Light Microscopy: 34:05, May 2012, can be accessed for free on the Wiley Online Library. more
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