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Super Resolution Microscopy with Cobolt Jive 561 nm -
Cobolt has introduced a higher power version of its Cobolt Jive 561nm. The laser is available on the 05-01 Series single frequency platform with an output power of 500 mW CW. 200 mW and 300 mW powers are also available on the same platform. Lower power versions (25 mW-150 mW) can be found on the 04-01 Series SLM platform. more
New Ways to Super-Resolution: Reversible Chemical Reactions Control Fluorescent States
Recently, light microscopy has been revolutionized by novel approaches that circumvent the diffraction barrier the resolution limit of optical microscopes. Most of these novel methods are based on light-controlled switching of the labels fluorescent states and therefore require use of additional or more intense laser excitation lines. To overcome these demands a new probe has been developed which is controlled by a reversible chemical reaction thereby reducing the demands on the microscopes.Limitations of Light Microscopy
moreJul. 29, 2011
Super-Resolution Microscopy: Physicist Building Advanced Microscope
Stephanie Meyer, a physicist specializing in optics, is bringing new capabilities to the University of Colorado Denver Anschutz Medical Campus by building an advanced, super-resolution microscope able to see some of the innermost workings of the cell. more
Restless Receptors - Dynamic Organization of Synapses
Synapses are the switch-points in our brain for information transmission, learning and memory. And there is evidence to suggest that changes and malfunctions in synapses are partly responsible for a number of neurological and psychological disorders. Neuroscientists already know a lot about how signals are transmitted from neuron to neuron. Yet many synaptic processes are still not fully understood. Dr. more
Award by the Bayer Science & Education Foundation
The winner of the 2011 Hansen Family Award has been decided. The Board of Trustees of the Bayer Science & Education Foundation and the Scientific Committee for this award have presented Professor Stefan W. Hell with the EUR 75,000 prize, which is one of Germany's most prestigious scientific accolades. His breakthroughs in the field of light microscopy provide insights into living cells and tissue that until recently would have been inconceivable. more
Come Together of Top Researchers
The sixteenth International Workshop on Single Molecule Spectroscopy and Ultra Sensitive Analysis in the Life Sciences from September 15 to 17, 2010, organised by PicoQuant, brought together international top researchers in the field of ultrasensitive optical detection and single molecule spectroscopy and microscopy. More than 150 participants enjoyed 45 highly up-to-date talks, including lectures given by renowned scientists like Steven A. Soper, Paul R. Selvin and Gerhard J. Schütz. During the poster session with more than 50 scientific posters participants discussed latest results. more
Launches for Bioimaging
ChromoTek has launched the bioimaging tools RFP-Trap and GFP-Booster. RFP-Trap is used to identify and pull down interaction partners of proteins tagged with RFP (red fluorescent protein) and GFP-Booster restores or increases the GFP (green fluorescent protein) signal in super-resolution microscopy. RFP-Trap is a tool for biochemical and cell biological analysis of RFP-tagged fusion proteins in pull down assays (a frequently used in vitro method used to determine physical interaction between two or more proteins). more
The Fast Track to Superresolution
The Leica TCS STED CW is the most uncomplex way to nanoscopy for research. The system resolves structures smaller than 80 nm - with purely optical methods. The depletion lasers and emission lasers in the visible spectral range enable researchers to use conventional dyes such as Alexa 488, FITC and Oregon Green and beyond that established fluorescence proteins such as YFP. Dynamics of sub-cellular processes can be imaged due to this flexibility and the system's ability of fast data acquisition. This means: live cell imaging below the diffraction limit. more
Tomographic Phase Microscopy
In visualizing transparent biological cells and tissues, the phase contrast microscope and its related techniques have been a cornerstone of nearly every cell biology laboratory. However, phase contrast methods are inherently qualitative and lack in 3-D imaging capability. We introduce a novel tomographic microscopy for quantitative three-dimensional mapping of refractive index in live cells and tissues using a phase-shifting laser interferometric microscope with variable illumination angle.more
Scientific Images
During the last decade microscopy has gone through a series of major improvements. The demand of microscopy technique has increased a lot and has brought the limits in optical resolution of light microscopes, as described by Ernst Abbe, to be extended by new technologies like 4Pi, STED [3], deconvolution and others. Scientists want to resolve small compartments and structures within a cell and but at the same time need to visualize a large field of view to be able to understand the complexity of biological organisms. more- Legal
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