May. 15, 2012

Explaining STED Microscopy by Boris Zarda from Leica Microsystems

Dr. Boris Zarda from Leica Microsystems shows the differences of confocal microscopy and STED microscopy on the basis of different examples. moreFeb. 09, 2012

STED Microscopy: Imaging the Living Mouse Brain

Using the STED microscopy developed by Stefan Hell, researchers at the Max Planck Institute for Biophysical Chemistry in Göttingen, Germany have, for the first time, managed to record detailed live images inside the brain of a living mouse. Captured in the previously impossible resolution of less than 70 nanometers, these images have made the minute structures visible which allow nerve cells to communicate with each other. moreDec. 27, 2011

Time-Gated STED Microscopy

Stimulated emission depletion microscopy has been used to overcome the diffraction limit of confocal fluorescence microscopy. By exploiting information present in the arrival time of fluorescence photons through time-gating, the resolution of STED microscopes can be improved significantly. The resolution improvement of this technique - termed "T-STED" - becomes most evident in CW-STED where the STED beam is of long duration compared to the lifetime of the fluorophore.

Introduction
moreSep. 02, 2011

Stefan Hell has Received The Körber Prize 2011

The Körber European Science Prize 2011 has been awarded to Prof. Dr. Dr. h. c. Stefan Hell of the Max Planck Institute for Biophysical Chemistry in Göttingen. moreJul. 19, 2011

Stefan Hell Receives the Körber Prize 2011

Prof. Dr. Dr. h. c. Stefan Hell of the Max Planck Institute for Biophysical Chemistry in Göttingen, Germany is to receive the 2011 Körber European Science Prize endowed with 750,000 € for his pioneering discoveries in the field of optics. Every year, the Körber Prize is awarded to an outstanding scientist working in Europe on particularly promising projects. The prizewinner is selected by an international trustee committee chaired by Prof. Dr. Peter Gruss, President of the Max Planck Society.
moreJun. 20, 2011

Single-Molecule FRET & Super-Resolution

In order for nanotechnology, photonics and single-molecule spectroscopy to meet, structures with defined molecular compositions with dimensions in the 1-100 nm range are required. With the aid of DNA, nanostructures were constructed that guide light in switchable directions using multistep FRET from an input dye to an output dye. The direction of FRET is controlled by a jumper dye. moreNov. 16, 2010

Confocal Nanoscopy Goes Multicolor

Scientists long to understand the architecture of life. They want to learn how biological structures are arranged in respect to one another. Do they co-localize within or are they excluded from the same superstructure? Does localization follow a special pattern and how does the overall arrangement reflect the biological function? Multicolor superresolution imaging allows these fundamental questions to be addressed by far-field fluorescence microscopy in unprecedented detail. moreFeb. 22, 2010

Perspectives of Superresolution in Neurobiology

Fully understanding the functionality and the complexity of the human central nervous system remains as one of the major open questions in modern science. The Drosophila neuromuscular junction (NMJ) is a widely used and acknowledged model for the analysis of synapse structure and assembly. When considering the size of a Drosophila NMJ synapse of about 500 nm in diameter it appears logical that in order to visualize its spatial architecture the resolution of image acquisition methods needs to be accordingly high. more