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Super-Resolution Microscopy

Apr. 10, 2014

Tracking the HI Virus with High-Resolution Microscopy

Experts from science and industry are working on new methods of microscopy and advancing microscopy technology to improve our ability to study and understand the molecular processes of HIV infection. Last year, the Federal Ministry of Education and Research (BMBF) approved approx. 4.6 million euros for this joint project called "Chemical Switches and Click Chemistry for High-resolution Microscopy". Meanwhile preparations for the start of the project are complete. The three-year "Switch Click Microscopy" project will involve scientists from Heidelberg University and Heidelberg University Hospital, the University of Würzburg, the European Molecular Biology Laboratory in Heidelberg as well as experts from four companies. The project coordinator is Prof. Dr. Dirk-Peter Herten, a member of the CellNetworks Cluster of Excellence who also works at the Institute for Physical Chemistry at Heidelberg University.
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Membrane Protein Localization by SpecON
Apr. 08, 2014

Membrane Protein Localization by SpecON

Spectrally coded optical nano-sectioning (SpecON) is a high-resolution microscopy technique that translates spatial (position) information of fluorescent markers into spectral (color) information providing a protein localization precision of up to 5-10 nm in live cells. The key element is a thin metal-dielectric coating on a microscope slide. The biocompatible design is such that the distance-dependent spectral "fingerprint" of fluorophores can be used to monitor their relative distance from the coating to study the positions and dynamics of key proteins in cell motility. more
3D Super-Resolution Microscopy: A New Understanding of Bacterial Division
Apr. 07, 2014

3D Super-Resolution Microscopy: A New Understanding of Bacterial Division

Scientists at EPFL have used a super-resolution microscopy technique to understand how bacteria divide. Their findings refute conventional models and can pave the way for the development of new antibiotics. The results have been published in PNAS.
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Structured Illumination Microscopy: Chemical Probe Profiles Live-cell Organelle Activity
Apr. 02, 2014

Structured Illumination Microscopy: Chemical Probe Profiles Live-cell Organelle Activity

A team of scientists from Pacific Northwest National Laboratory synthesized a chemical activity-based probe (ABP) that can provide new information about how living cells function. The new ABP is designed to enter a living cell without interacting with anything until it enters a specific organelle: the lysosome. This proof-of-concept ABP then labels only functionally active enzymes called cathepsins, which are cysteine proteases, in the lysosome. Using proteomics and super-resolution microscopy to view these labeled enzymes, the scientists now are able to see organellar activity. Their work, which demonstrates the ability to manipulate chemistry to better understand biology, has been published in Angewandte Chemie International Edition.
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How to Set Up a 3D-SIM Microscope (User Manual)
Apr. 01, 2014

How to Set Up a 3D-SIM Microscope (User Manual)

In the past two decades, light microscopy has seen a tremendous improvement with super-resolution techniques. Many of the super-resolution microscopes (3D-SIM, STED, and PALM/STORM) are now available in a commercial solution, and are entering labs and facilities worldwide. This offers an important step forward in the field of research in biology, but when not used in optimal conditions, those powerful techniques can give rise to artefacts. Here we focus on parameters that can deeply influence the image quality for 3D-SIM and the next challenges for this technique. more
Andor´s Zyla 4.2 – Offering the Highest QE Available from sCMOS Technology
Mar. 31, 2014

Andor´s Zyla 4.2 – Offering the Highest QE Available from sCMOS Technology

Zyla sCMOS has proven a superb camera choice for the biologist and microscopist.

Many simply see the Zyla as an amazing value, superb price/performance 'workhorse' camera with which to replace their existing interline CCD and upgrade the performance of their fluorescence microscope. more
Winners of the GE Healthcare 2013 Cell Imaging Competition
Mar. 21, 2014

Winners of the GE Healthcare 2013 Cell Imaging Competition

GE Healthcare Life Sciences announced Vanessa Auld from Canada, Martin Barr from Ireland and Graham Wright from Singapore as the winners of the GE Healthcare 2013 Cell Imaging Competition. more
Trends in Microscopy 2014
Mar. 11, 2014

Trends in Microscopy 2014

The conference "Trends in Micoscopy 2014" will take place from March 27 -29 in Freiburg, Germany. The conference brings together leading high-end microscope techniques developers and users from Germany with their world-wide colleagues.

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Super-Resolution Research Highlight
Feb. 13, 2014

Super-Resolution Research Highlight

Joint research on super-resolution microscopy from the National Physical Laboratory (NPL) and the University of Cambridge has been selected as a highlight of 2013 by the Journal of Optics, making it freely available to view online.
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Medical Imaging: Breakthrough in Fiber-optical Two-photon Fluorescence Endoscopy
Jan. 25, 2014

Medical Imaging: Breakthrough in Fiber-optical Two-photon Fluorescence Endoscopy

A breakthrough technique for super-resolution 3D medical imaging of living cells has been developed by researchers at Swinburne University of Technology. The new technique potentially could aid in minimally-invasive surgery and the early detection of cancer. The research is published in Scientific Reports.
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