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Super-Resolution Microscopy

New Fluorescence Probes for Live-Cell Imaging
Oct. 30, 2014

New Fluorescence Probes for Live-Cell Imaging

We review here a new generation of far-red fluorescent probes for live-cell imaging, which are based on a novel cell-permeable silicon rhodamine (SiR) dye. These probes combine a variety of desirable features, such as excellent selectivity, fluorogenicity, high brightness and low cytotoxicity, rendering them ideal probes for conventional and state-of-the-art super-resolution microscopy. Application of these new probes in combination with STED microscopy revealed for the first time the nine-fold symmetry of the centrosome and confirmed the spatial organization of actin in the axon of cultured neurons in living cells.
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Microscopy Research & Technique: Read Articles of Nobel Prize Winner Stefan Hell
Oct. 27, 2014

Microscopy Research & Technique: Read Articles of Nobel Prize Winner Stefan Hell

Microscopy Research & Techniques: Stefan Hell, 2014 Chemistry Nobel prize co-winner
View Professor Hell´s foreward and collection of research articles published in the journal - free until the end of December 2014
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High-Resolution Microscopy: New Membrane Probe to Analyze Cellular Structures
Sep. 26, 2014

High-Resolution Microscopy: New Membrane Probe to Analyze Cellular Structures

In the past two decades, super-resolution microscopy has been one of the fastest evolving fields through many technical improvements. However, the development of new labeling tools, probes and their biological application, is mostly lagging behind the technical capabilities. Most recently, Prof. Silvio O. Rizzoli from the Cluster of Excellence and DFG- Research Center for Nanoscale Microscopy and Molecular Physiology of the Brain (CNMPB) has developed together with his team a new technique that expands the benefit of super- resolution microscopy to study biological questions. This method contributes to understand on how cells renew, distribute and transport their molecular and subcellular components. The new technique was published the Journal of Cell Biology.
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Airyscanning
Aug. 24, 2014

Airyscanning

Confocal laser scanning microscopes (CLMs) are renowned for their sectioning capability. This feature is enabled by the use of pinhole, which rejects out-of-focus light. Less appreciated, on the other hand, is the gain in lateral resolution by this type of microscopes for one obvious reason. more
Labeling & Nanoscopy Conference
Aug. 11, 2014

Labeling & Nanoscopy Conference

The conference Labeling & Nanoscopy will take place from 24-26 September 2014 in Heidelberg, Germany. This conference brings together experts in fluorescence and labeling with those designing and applying new concepts of far-field optical nanoscopy.
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Super-Resolution Microscopy
Aug. 07, 2014

Super-Resolution Microscopy

In the year 2008 Nature Methods has selected super-resolution microscopy or nanoscopy as "method of the year" [1]. Since that time super-resolution microscopy has emerged from specialized physics laboratories and become a powerful tool for biologists. The field of application is constantly growing and since recently even expanding in the axial dimension for single molecule localization microscopy. The process to get high quality super-resolution images can be divided in three important steps: sample preparation, image acquisition and image processing. Especially the first step has to be considered with a lot of care, since higher resolving power demands more stringent sample preparation.
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Carl Zeiss´s LSM 880 with Airyscan: Confocal Laser Scanning Microscope for Super-resolution Microscopy
Jul. 31, 2014

Carl Zeiss´s LSM 880 with Airyscan: Confocal Laser Scanning Microscope for Super-resolution ...

Carl Zeiss Microscopy has released LSM 880 with Airyscan. The confocal laser scanning microscope enables fast and high sensitivity super-resolution microscopy in x, y and z, and high image-acquisition speed in one system.
With the new confocal technology, users achieve a 1.7x higher resolution in all spatial dimensions, 140 nm laterally and 400 nm axially. The improved sensitivity leads to better image quality and increased speed. The whole imaging process is possible with standard sample preparation and labeling protocols. more
Jul. 31, 2014

Bruker Announces Acquisition of High-Speed, 3D Super-Resolution Fluorescence Microscopy Company ...

Bruker announced that it has acquired Vutara, a provider of high-speed, three-dimensional (3D), super-resolution fluorescence microscopy for life science applications. Transaction details were not disclosed. Vutara's estimated revenue for the full year 2014 is expected to be approximately $-US 2 million.
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Multicolor Super-Resolution Fluorescence Imaging
Jul. 07, 2014

Multicolor Super-Resolution Fluorescence Imaging

Stochastic optical reconstruction microscopy (STORM) has the potential to increase the resolution in fluorescence light microscopy up to tenfold. This helps scientists to get new insights into biological processes and structural details. For STORM fluorophores are utilized that show a blinking behavior [1] switching between a fluorescent and a nonfluorescent state. This was initially described for pairs of cyanine dyes, one activator dye and one reporter dye [1] in close proximity to each other. For multicolor STORM the channels are separated by using different activator dyes while the reporter dye is the same in each channel [2].
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Lensless Imaging without Spectral Bandwidth Limitations or Sample Requirements
Jun. 26, 2014

Lensless Imaging without Spectral Bandwidth Limitations or Sample Requirements

The overwhelming majority of past and present imaging systems use a lens to focus the subject of interest, even today's super-resolution light microscopes that breach the diffraction limit through ingenious experimental methods. Lensless imaging offers the prospect of a radical improvement in resolution by reconstructing a high-resolution image of an object from one or more diffraction patterns.
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