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Transmission Electron Microscopy

Transmission Electron Microscopy: Visualizing for the First Time the Open Form of the Cohesin Complex
Nov. 24, 2014

Transmission Electron Microscopy: Visualizing for the First Time the Open Form of the Cohesin ...

The cohesin molecule ensures the proper distribution of DNA during cell division. Scientists at the Research Institute of Molecular Pathology (IMP) in Vienna can now prove the concept of its carabiner-like function by visualizing for the first time the open form of the complex by using a Transmission Electron Microscope. The results have been published in Science.
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Examining Materials in Liquids Using Transmission Electron Microscopy
Sep. 08, 2014

Examining Materials in Liquids Using Transmission Electron Microscopy

Accurately examining materials in liquids using electron microscopy is a difficult task for scientists, as electron beams perturb the sample and induce artifacts. This is especially true when using in situ liquid transmission electron microscopy (TEM) and scanning TEM (STEM) to probe nanomaterials. Scientists at Pacific Northwest National Laboratory (PNNL) and University of California, Davis demonstrated that in in situ liquid experiments, the choice of electron beam energy has a strong effect that goes far beyond merely increasing the concentration of reducing radicals. They also found that when compared to solid samples, radicals formed in the liquid phase are more mobile, and ultimately dictate the choice of TEM imaging mode.
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Dual-Beam Microscope Will Allow High-Resolution Look Inside Cells
Aug. 15, 2014

Dual-Beam Microscope Will Allow High-Resolution Look Inside Cells

The University of California, San Diego's Nanofabrication Cleanroom Facility (Nano3) is the first institution to obtain a novel FEI Scios dual-beam microscope, with an adaptation for use at cryogenic temperatures. The new microscope will enable research among a highly diverse user base, ranging from materials science to structural and molecular biology.
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TEM Sample Preparation of Nanoparticles in Suspensions
Jul. 07, 2014

TEM Sample Preparation of Nanoparticles in Suspensions

Nanomaterials are of significant economic interest with a global market value of roughly 20 billion €, which is expected to rise to 2 trillion € by 2015 [1]. For commercial use, the European Union provides the following recommended definition: "‘Nanomaterial' means a natural, incidental or manufactured material containing particles, in an unbound state or as an aggregate or as an agglomerate and where, for 50% or more of the particles in the number size distribution, one or more external dimensions is in the size range 1 nm-100 nm" [2].
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New Book: Ultrastructure Atlas of Human Tissues
Jun. 30, 2014

New Book: Ultrastructure Atlas of Human Tissues

Ultrastructure Atlas of Human Tissues presents a variety of scanning and transmission electron microscope images of the major systems of the human body. Photography with the electron microscope records views of the intricate substructures and microdesigns of objects and tissues, and reveals details within them inaccessible to the naked eye or light microscope. Many of these views have significance in understanding normal structure and function, as well as disease processes. This book offers a unique and comprehensive look at the structure and function of tissues at the subcellular and molecular level, an important perspective in understanding and combating diseases.
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Focused Low Energy-Argon Ion Milling
May. 19, 2014

Focused Low Energy-Argon Ion Milling

High energy focused ion beam (FIB) milling produces ion-induced damage into TEM samples and a certain amount of Ga ions implantation cannot be avoided. Additional polishing of FIB lamellae at low voltages can damage the sample further. To overcome these disadvantages, a low-energy Ar+-milling of a FIB lamellae can be applied [1,2]. In this work, we focus on TEM sample preparation of different thin films and interface structures using a combination of FIB with a focused low-energy Ar+-polishing.
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FEI Company Introduces Talos TEM
Dec. 25, 2013

FEI Company Introduces Talos TEM

The Talos TEM from FEI Company combines high-resolution and high-throughput TEM imaging with fast, precise and quatitative energy dispersive x-ray (EDX) analysis.
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3D Dynamic Imaging of Soft Materials
Oct. 08, 2013

3D Dynamic Imaging of Soft Materials

Through a combination of transmission electron microscopy (TEM) and their own unique graphene liquid cell, researchers at the U.S. Department of Energy (DOE)'s Lawrence Berkeley National Laboratory (Berkeley Lab) have recorded the three-dimensional motion of DNA connected to gold nanocrystals (see movie). This is the first time TEM has been used for 3D dynamic imaging of so-called soft materials. The results were published in the journal Nano Letters.
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Exploring Teeth by Analytical (S)TEM
Sep. 23, 2013

Exploring Teeth by Analytical (S)TEM

Mineralized human dental tissues were studied by advanced analytical transmission electron microscopy (TEM) techniques. The Ca/P and Mg/P at % ratios were determined employing energy dispersive X-ray spectroscopy (EDX) measurements. Significantly lower Ca/P and higher Mg/P at % ratios were measured in peritubular dentine compared to intertubular dentine, suggesting the incorporation of Mg in the hydroxylapatite lattice by substituting Ca. Improvement of TEM sample preparation is demonstrated. more
In Situ Molecular Microscopy
Jul. 31, 2013

In Situ Molecular Microscopy

We present a novel approach for visualizing viral assemblies in liquid using transmission electron microscopy (TEM). We utilize a microfluidic chamber that fits within a TEM specimen holder and while inserted in the column, is completely isolated from the vacuum. This configuration proves suitable for imaging and reconstructing viral complexes in solution at 2.5-nanometer resolution. more
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