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Transmission Electron Microscopy

TEM Sample Preparation of Nanoparticles in Suspensions
Jul. 07, 2014

TEM Sample Preparation of Nanoparticles in Suspensions

Nanomaterials are of significant economic interest with a global market value of roughly 20 billion €, which is expected to rise to 2 trillion € by 2015 [1]. For commercial use, the European Union provides the following recommended definition: "‘Nanomaterial' means a natural, incidental or manufactured material containing particles, in an unbound state or as an aggregate or as an agglomerate and where, for 50% or more of the particles in the number size distribution, one or more external dimensions is in the size range 1 nm-100 nm" [2].
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New Book: Ultrastructure Atlas of Human Tissues
Jun. 30, 2014

New Book: Ultrastructure Atlas of Human Tissues

Ultrastructure Atlas of Human Tissues presents a variety of scanning and transmission electron microscope images of the major systems of the human body. Photography with the electron microscope records views of the intricate substructures and microdesigns of objects and tissues, and reveals details within them inaccessible to the naked eye or light microscope. Many of these views have significance in understanding normal structure and function, as well as disease processes. This book offers a unique and comprehensive look at the structure and function of tissues at the subcellular and molecular level, an important perspective in understanding and combating diseases.
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Focused Low Energy-Argon Ion Milling
May. 19, 2014

Focused Low Energy-Argon Ion Milling

High energy focused ion beam (FIB) milling produces ion-induced damage into TEM samples and a certain amount of Ga ions implantation cannot be avoided. Additional polishing of FIB lamellae at low voltages can damage the sample further. To overcome these disadvantages, a low-energy Ar+-milling of a FIB lamellae can be applied [1,2]. In this work, we focus on TEM sample preparation of different thin films and interface structures using a combination of FIB with a focused low-energy Ar+-polishing.
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FEI Company Introduces Talos TEM
Dec. 25, 2013

FEI Company Introduces Talos TEM

The Talos TEM from FEI Company combines high-resolution and high-throughput TEM imaging with fast, precise and quatitative energy dispersive x-ray (EDX) analysis.
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3D Dynamic Imaging of Soft Materials
Oct. 08, 2013

3D Dynamic Imaging of Soft Materials

Through a combination of transmission electron microscopy (TEM) and their own unique graphene liquid cell, researchers at the U.S. Department of Energy (DOE)'s Lawrence Berkeley National Laboratory (Berkeley Lab) have recorded the three-dimensional motion of DNA connected to gold nanocrystals (see movie). This is the first time TEM has been used for 3D dynamic imaging of so-called soft materials. The results were published in the journal Nano Letters.
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Exploring Teeth by Analytical (S)TEM
Sep. 23, 2013

Exploring Teeth by Analytical (S)TEM

Mineralized human dental tissues were studied by advanced analytical transmission electron microscopy (TEM) techniques. The Ca/P and Mg/P at % ratios were determined employing energy dispersive X-ray spectroscopy (EDX) measurements. Significantly lower Ca/P and higher Mg/P at % ratios were measured in peritubular dentine compared to intertubular dentine, suggesting the incorporation of Mg in the hydroxylapatite lattice by substituting Ca. Improvement of TEM sample preparation is demonstrated. more
In Situ Molecular Microscopy
Jul. 31, 2013

In Situ Molecular Microscopy

We present a novel approach for visualizing viral assemblies in liquid using transmission electron microscopy (TEM). We utilize a microfluidic chamber that fits within a TEM specimen holder and while inserted in the column, is completely isolated from the vacuum. This configuration proves suitable for imaging and reconstructing viral complexes in solution at 2.5-nanometer resolution. more
Morada G2 TEM camera from Olympus
Jul. 01, 2013

Morada G2 TEM camera from Olympus

The 11 Megapixel Morada G2 TEM camera from Olympus Soft Imaging Solutions offers an extremely large field of view combined with maximum resolution, high frame rates more
CLEM on Ultrathin Sections
Jun. 18, 2013

CLEM on Ultrathin Sections

Correlative light and electron microscopy (CLEM) combines the versatility of fluorescence microscopy (FLM) with the spatial resolution of transmission electron microscopy (TEM). For the analysis of tissues single resin or cryo-sections are incubated with fluorochrome- and gold-labeled probes. Areas of interest are selected at the FLM, and analyzed in the TEM at high resolution. This way, fluorescence is directly correlated to subcellular structures and/or corresponding immunogold signals.
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Ted Pella Introduces Pelco Graphene TEM Support Films
May. 06, 2013

Ted Pella Introduces Pelco Graphene TEM Support Films

Ted Pella, Inc has announced the availability of the new research-ready Pelco graphene TEM support films. The films are supported by lacey carbon film on a 300 mesh copper grid. The single, continuous graphene sheet covers the entire 300 mesh area of the TEM grid. This creates a usable area of around 75% of the TEM grid, leaving plenty of space for specimens or experiments. The Pelco graphene TEM support films with the large area continuous film can be used directly out of the box. more
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