Oct. 11, 2009

A Spectral Revolution

By removing out-of-focus light from above and below the focal plane, particularly in specimens >20 µm thick, the Olympus Disk Scanning Unit (DSU) uniquely delivers confocal-like images with excellent contrast and resolution compared to conventional wide-field microscopy. To reject the out-of-focus light, the DSU places a spinning disk, with an alternating pattern of vertical and horizontal slits, in the confocal plane of the microscope. moreNov. 01, 2008

Wide-field CARS-Microscopy

Coherent anti-Stokes Raman scattering (CARS) microscopy is a branch of nonlinear microscopy that allows chemical imaging of targeted vibrational transitions in unstained samples. A resonantly enhanced blue-shifted CARS signal is generated from NIR or visible light, thus the method is more sensitive than normal Raman microscopy and offers better resolution than IR microscopy. CARS microscopes are mostly set up as confocal scanning microscopes, but wide-field approaches are possible as well.

Brief Introduction to CARS-microscopy
moreNov. 01, 2007

Wide-field CARS-Microscopy: Functional Imaging at a Glimpse

Wide-field CARS-Microscopy: Functional Imaging at a Glimpse. Coherent anti-Stokes Raman scattering (CARS) microscopy is a branch of nonlinear microscopy that allows chemical imaging of targeted vibrational transitions in unstained samples. A resonantly enhanced blueshifted CARS signal is generated from NIR or visible light, thus the method is more sensitive than normal Raman microscopy and offers better resolution than IR microscopy. CARS microscopes are mostly set up as confocal scanning microscopes, but wide-field approaches are possible as well. moreApr. 01, 2007

Photoswitching: Wide-field Subdiffraction RESOLFT Microscopy

Photoswitching: Wide-field Subdiffraction RESOLFT Microscopy. M. Schwentker and her co-workers describe the use of the principle of reversible saturable optical (fluorescence) transitions (RESOLFT) in a wide-field microscopy arrangement for subdiffraction imaging. To achieve this, the saturated transition of a photoswitchable fluorescent protein to its dark state is forced by the projection of a line pattern above the resolution limit that leaves only subdiffraction distances between adjacent lines in which the fluorescent form of the protein can be detected. Thus, a resolution of approx. more