Dr. Boris Zarda from Leica Microsystems shows the differences of confocal microscopy and STED microscopy on the basis of different examples.
Stimulated emission depletion microscopy, or STED microscopy, is a fluorescence microscopy technique that uses the non-linear de-excitation of fluorescent dyes to overcome the resolution limit imposed by diffraction with standard confocal laser scanning microscopes and conventional far-field optical microscopes. Therefore, it is also described as a form of super-resolution microscopy.
More information on super-resolution microscopy:
http://www.leica-microsystems.com
Related Articles :
Keywords: CW-STED Leica Microsystems STED microscopy Stimulated Emission Depletion Microscopy Super-Resolution Microscopy T-STED
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Imaging & Microscopy Issue 4 , 2012 as free epaper or pdf download
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