Oct. 07, 2014

Lensless Microscopy of Cell Cultures - Video to Figure 1

Video to figure 1: The in vitro cell growth of a fibroblast L-929 culture was recorded every 10 minutes over 7 days in more than 1000pictures and visualized as a time-lapse video (fig. 1).

The lensless Cell-Microscope combines holographic imaging of cells with a thermoelectric cooling system and automated cell segmentation for live cell imaging inside the incubator. Using holography instead of optical focusing enables to build a cost-efficient, robust and compact microscope. The cooling of the CMOS camera defines the local temperature of the integrated cell culture chamber. Thousands of cells can be imaged, segmented, counted and tracked continuously within the large field of view.

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Dipl.-Ing. Moritz Hubl
Fraunhofer IZM
Medical Microsystems
Berlin, Germany


Fraunhofer-Institut für Zuverlässigkeit und Mikrointegration IZM
Gustav-Meyer-Allee 25
13355 Berlin

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