Chapter 5: FISH Spot Detection in Human Spermatozoids
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In this chapter we will detect spots (chromosomes) inside a larger structure (spermatozoid). The dataset has four channels that we will split and process - initially by hand and then we will create macros of increasing complexity that performs all of the steps automatically. You will learn how to work with image masks, analyse particles, apply watershed segmentation, and create color-coded overlay images that illustrate the results of the image analysis
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