Chapter 7: Quantitative Evaluation of Multicellular Movements in Drosophila Embryo
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This chapter focuses on strategies to track cells within the mono-layer epithelium of a Drosophila embryo.
This is a challenging task since the cells cannot be considered as spots and tracked by classical spot trackers as their size and geometry is very variable.
We will rely on the strength of ImageJ to build an interactive script to segment the cells in the movies and enable user cross-checking and correction.
Then we will switch to Matlab to import the results from the segmentation, perform cell tracking, extract statistics on geometry and position of the cells and plot the results.
The tracking of the objects is based on their overlap across frames and we will introduce important concepts about particle connectivity and mathematical morphology to build a robust cell tracker.
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The material used in this chapter is available from the "Read Whitepaper" link next to the picture on top of this article.
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