Apr. 01, 2016

Combining the MicroTime 200 with the Bruker BioScope Catalyst AFM for Multi-parameter Cell Imaging

  • Combination of Bruker's AFM with MicroTime 200.Combination of Bruker's AFM with MicroTime 200.
  • Combination of Bruker's AFM with MicroTime 200.
  • Fluorescence lifetime image (A) and 3D topography image (B) of a cell sample, that were recorded in a synchronized manner.

Combination of Atomic Force Microscopy (AFM) with single-molecule-sensitive fluorescence microscopy grants fascinating insights into the structure, dynamics, and interactions in cells. The AFM part reveals structural information of macromolecular complexes on the nanometer scale, while fluorescence lifetime data facilitates identification of their constituent parts. Thus, the combination of both techniques in a single instrument opens up new prospects in live cell imaging, without sacrificing optical image quality. PicoQuant's MicroTime 200 platform can be interfaced to a series of AFMs from Bruker, JPK, and Asylum Research and maintains its full range of capabilities such as super-resolution via Stimulated Emission Depletion (STED).

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