Mar. 03, 2016


Super-Resolution Imaging with a Confocal Microscope

Since the invention of the microscope, there has been continual discussion about the possibility of showing more detailed features of specimens as compared to just magnifying them. Ernst Abbe was the first to explore the issue in depth and found there was a limit to the size of details visible in ordinary light microscopes [1].

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[1] Abbe EK: Beiträge zur Theorie des Mikroskops und der mikroskopischen Wahrnehmung, Archiv für Mikroskopische Anatomie 9 (1) 413–468 (1873)
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[4] Sparrow CM: On spectroscopic resolving power, Astrophysical Journal 44:76 (1916)
[5] Houston, WV: A compound interferometer for fine structure work, Physical Review, 29 (3), pp 478-484 (1927)
[6] Minsky M: Memoir on Inventing the Confocal Scanning Microscope, Scanning 10, 128-138 (2011)
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[9] Borlinghaus RT, Birk H and Schreiber F: Detectors for Sensitive Detection: HyD, in Current Microscopy Contributions to Advances in Science and Technology (A Méndez-Vilas, Ed) Formatex (2012)
[10] Parœmiologia Anglo-Latina, Clarke J (editor), London, 1639
[11] Scientific Volume Imaging: Image Restoration vs Image Enhancement retrieved Jan 19th 2016
[12] Scientific Volume Imaging retrieved Nov 16th, 2015
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Borlinghaus RT & Kappel C
Leica Microsystems Mannheim


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