A New Laser Concept for Nonlinear Microscopy
Individual Laser Lines for Live-Cell Multicolor 2-Photon Imaging
- Fig. 1: The two-color fiber laser FemtoFiber dichro design bioMP has two output wavelengths 780 nm (100 mW) and 1030 nm (200 mW) and can additionally generate a ´virtual´wavelength (888 nm) at the sample. As both wavelengths originate from the same oscillator, the pulses are always perfectly synchronized. In order to achieve perfect temporal overlap of both pulse trains at the sample one of the two wavelengths can be shifted by Δt in time.
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The laser presented here follows a completely new approach for 2-photon microscopy. The all-fiber laser emits sub-100-fs pulses at 780 nm and 1030 nm through one aperture and additionally generates a ‘virtual' 2-photon excitation line at 888 nm at the sample. The system allows exciting all conventional fluorescent markers including GFP. Furthermore, it enables live-cell multicolor 2-photon imaging with up to three excitation wavelengths simultaneously.
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