rapidFLIM: Fast Imaging Technique Redefining the Standards for Dynamic FLIM Imaging
- Diffusion of dye-labeled beads in water, imaged with three frames per second. A selection of frames from the video is shown.
- FastFLIM images of mammalian cells expressing a plant transcription factor fused to Venus. We show here the first and last frame of the video. Sample courtesy of Stefanie Weidtkamp-Peters, CAI, University of Düsseldorf, Germany
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The rapidFLIM method exploits recent advances in Time-Correlated Single Photon Counting (TCSPC) electronics, where ultra-short dead times allows for imaging fast, dynamic processes via Fluorescence Lifetime Imaging (FLIM). This new approach allows for fast FLIM acquisition with up to several full frames per second for imaging of dynamic processes (e.g., protein interaction, chemical reaction, or ion flux), highly mobile species (e.g., mobility of cell organelles or particles, cell migration), and investigating FRET dynamics. More than 10 frames per second can be acquired, depending on sample brightness and image size.
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