Simultaneous Multi-color, Multiphoton Fluorophore Excitation using Dual-color Fiber Lasers
- TPEF fluorescence map excited simultanously at different wavelength emitted by the FemtoFiber dichro design bioMP. Both beams share the same beam path to guarantee a spatial overlap at the sample. By tuning the respective temporal delay of both beams to zero a virtual excitation at 890 nm takes place exciting also the GFP neutrophil cell as shown in the inset.
- Read Whitepaper
Advanced microscopy techniques provide crucial insights for a deeper understanding of biological and chemical processes. Due to its nonlinear excitation character, multiphoton imaging (e.g. TPEF, SHG, THG) enables a deeper penetration depth and higher spatial resolution than conventional techniques. Here we present a novel approach to two-photon excitation at various wavelengths, i.e. 2-color 2-photon excitation, simultaneously using Toptica’s FemtoFiber dichro design bioMP.