Switching to a New Perspective
Expanding Analysis Options through Inverted Confocal Raman Imaging
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Confocal Raman imaging is a well-established technique for investigating the chemical and molecular compositions of solid and liquid samples. Its varied fields of application in academia and industry range from materials and surface sciences to environmental and geo sciences to life sciences and pharmaceutics.
The array of measured sample characteristics requires versatile and adaptive analyzing capabilities. For confocal Raman microscopy, a conventional upright microscope geometry is usually used, where the microscope stage is located beneath the objective and the sample is observed from above. However, some samples are not suitable for examination with an upright microscope as they require a specific environment, such as biological cells in culture, or due to large sample dimensions. In these cases, an inverted microscope setup in which the microscope stage is located above the objective and the sample is observed from below is preferable for confocal Raman imaging. Thus inverted confocal Raman imaging opens up new opportunities for the investigation of aqueous or bulky samples. In the following, we will introduce the inverted confocal Raman imaging technique and provide some application examples.
1WITec GmbH, Ulm, Germany
Dr. Sonja Breuninger
Technical Marketing & PR
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