Jun. 18, 2018
Applications

Switching to a New Perspective

Expanding Analysis Options through Inverted Confocal Raman Imaging

Confocal Raman imaging is a well-established technique for investigating the chemical and molecular compositions of solid and liquid samples. Its varied fields of application in academia and industry range from materials and surface sciences to environmental and geo sciences to life sciences and pharmaceutics.

The array of measured sample characteristics requires versatile and adaptive analyzing capabilities. For confocal Raman microscopy, a conventional upright microscope geometry is usually used, where the microscope stage is located beneath the objective and the sample is observed from above. However, some samples are not suitable for examination with an upright microscope as they require a specific environment, such as biological cells in culture, or due to large sample dimensions. In these cases, an inverted microscope setup in which the microscope stage is located above the objective and the sample is observed from below is preferable for confocal Raman imaging. Thus inverted confocal Raman imaging opens up new opportunities for the investigation of aqueous or bulky samples. In the following, we will introduce the inverted confocal Raman imaging technique and provide some application examples.

Affiliation
1WITec GmbH, Ulm, Germany

Contact
Dr. Sonja Breuninger

Technical Marketing & PR
WITec GmbH
Ulm, Germany
Sonja.Breuninger@witec.de

References
[1] J. Toporski, T. Dieing, O. Hollricher (Editors), Confocal Raman Microscopy, 2nd Edition, Springer Series in Surface Sciences (66), Springer International Publishing AG (2018)
[2] P. Heraud, K. M. Marzec, Q. H. Zhang, W. S. Yuen, J. Carroll, B. R. Wood, Label-free in vivo Raman microspectroscopic imaging of the macromolecular architecture of oocytes. Scientific reports (2017) DOI: 10.1038/s41598-017-08973-0.
[3] H. Abramczyk, J. Surmacki, M. Kopec, A. K. Olejnik, A. Kaufman-Szymczyk, K. Fabianowska-Majewska, Epigenetic changes in cancer by Raman imaging, fluorescence imaging, AFM and scanning near-field optical microscopy (SNOM).

Acetylation in normal and human cancer breast cells MCF10A, MCF7 and MDA-MB-231. Analyst (2016) DOI: 10.1039/c6an00859c.
[4] K. Majzner, S. Chlopicki, M. Baranska, Lipid droplets formation in human endothelial cells in response to polyunsaturated fatty acids and 1-methyl-nicotinamide (MNA); confocal Raman imaging and fluorescence microscopy studies. Journal of Biophotonics (2016) DOI: 10.1002/jbio.201500134.
[5] X.-P. Wang, Y. Zhang, M. Konig, E. Papadopoulou, B. Walkenfort, S. Kasimir-Bauer, A. Bankfalvi, S. Schlucker, iSERS microscopy guided by wide field immunofluorescence: analysis of HER2 expression on normal and breast cancer FFPE tissue sections. Analyst (2016) DOI: 10.1039/C6AN00927A.
[6] C. Kallepitis, M. S. Bergholt, M. M. Mazo, V. Leonardo, S. C. Skaalure, S. A. Maynard, M. M. Stevens, Quantitative volumetric Raman imaging of three dimensional cell cultures. Nature Communications (2017) DOI: 10.1038/ncomms14843
[7] K. Czamara, K. Majzner, A. Selmi, M. Baranska, Y. Ozaki, A. Kaczor, Unsaturated lipid bodies as a hallmark of inflammation studied by Raman 2D and 3D microscopy. Scientific reports (2017) DOI: 10.1038/srep40889

Contact

WITec GmbH
Lise-Meitner-Str. 6
89081 Ulm
Germany
Phone: +49 731 140 700
Telefax: +49 731 140 70 200

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