Unmask the Hidden without Probes: CARS
Imaging of Delicate Samples with Coherent Anti-Stokes Raman Scattering Microscopy
- Fig. 1: (left) Study of localization and grade of accumulation of lipids in different types of macrophages: Lipids are displayed in red, actin in green, 63x objective. Courtesy: Alba Alfonso Garcia and Jeff Suhalim, Potma Lab, University of California, USA. Cells: Dr. Adelheid Kratzer, Department of Medicine Division of Pulmonary and Critial Care, University of Colorado.
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Fluorescence microscopy assumed a pivotal role in cell biology once it was possible to stain cell components selectively by fluorescing dyes. One of the first explorers of targeted stainings, Paul Ehrlich, had the idea that something that stains specifically should also kill specifically - which was associated with the term "magic bullet", the essential idea of chemotherapy. His group discovered Salvarsan, a tailored drug against syphilis - though not specific enough not to cause substantial side effects. Screening many fluorescent dyes led to a long list of stainings which are used in histology, including dyes like DAPI or hematoxylin and eosin.
Dr. Rolf T. Borlinghaus, Scientific Advisor
Dr. Stefanie Degenhartt, Scientific Advisor
Leica Microsystems CMS GmbH
Christine Ludwig (contact via email request button)
Leica Microsystems GmbH