Correlative Microscopy Using SEM Array Tomography
Burel et al., recently presented an optimized scanning electron microscopy (SEM) workflow for volumetric Array Tomography for asymmetric samples. A modified diamond knife was modified to simplify serial section arrays acquisition with minimal artifacts. After array acquisition, the arrays are transferred to a glass coverslip or silicon wafer support. Using light microscopy, the authors rapidly screen the arrays for initial recognition of global anatomical features (organs or body traits). Then, using SEM, an in-depth study of the cells and/or organs of interest was performed. This method can be used to address questions in cell and developmental biology that require the efficient identification of a labeled cell or organelle.
Agnes Burel et al.: A targeted 3D EM and correlative microscopy method using SEM array tomography, Development (2018) DOI:10.1242/dev.160879