Creating Serial Ultrathin Sections for SEM Array Tomography
In a paper by Taro Koike et al., a new “Device for Ribbon Collection for Array Tomography with Scanning Electron Microscopy” is presented. Serial ultrathin sections in the ribbon state (ribbons) are mounted on a solid substrate and observed by scanning electron microscopy (SEM). The method may also be used in conjunction with post-embedding immunocytochemistry. The authors build an inexpensive laboratory-made device that mounts ribbons by pulling a nylon fishing line and lifting the substrate up from the water in a knife boat.
The second publication "Methods for array tomography with correlative light and electron microscopy" is a review also Koike and Yamada and gives an overview on preparation of specimens for array tomography, including ribbon collection and the staining method, and the adaptability for correlative light and electron microscopy.
Taro Koike et al.: A Device for Ribbon Collection for Array Tomography with Scanning Electron Microscopy, Acta Histochem Cytochem., 50(5): 135–140 (24 Oct 2017) DOI: 10.1267/ahc.17013
Taro Koike and Hisao Yamada: Methods for array tomography with correlative light and electron microscopy, Medical Molecular Morphology (May 2018) DOI: 10.1007/s00795-018-0194-y