Nov. 03, 2016

Read & Win: Fundamentals of Light Microscopy and Electronic Imaging

  • Fundamentals of Light Microscopy and Electronic ImagingFundamentals of Light Microscopy and Electronic Imaging

Fundamentals of Light Microscopy and Electronic Imaging, Second Edition provides a coherent introduction to the principles and applications of the integrated optical microscope system, covering both theoretical and practical considerations. It expands and updates discussions of multi-spectral imaging, intensified digital cameras, signal colocalization, and uses of objectives, and offers guidance in the selection of microscopes and electronic cameras, as well as appropriate auxiliary optical systems and fluorescent tags.

Win the book!
To have a chance of winning the book read Issue 4, 2016 of Imaging & Microscopy (page 14 ). As a subscriber you could read the issue already online or order you own copy (as a free trial copy). Take part in our competition and send your answer to with the subject line Read & Win. All correct answers will be entered in a prize draw and the lucky winner will receive a copy of "Fundamentals of Light Microscopy and Electronic Imaging".
Closing date: March 22, 2017.

Douglas B. Murphy - Doug Murphy directed core facilities in microscopy and imaging for 8 years at the HHMI Janelia Research Campus in Ashburn, Virginia, with emphasis on confocal imaging, image montage of extended sections, and live cell imaging.   Prior to that he was a Professor of Cell Biology (1978-2006) at the Johns Hopkins Medical School in Baltimore, MD, where he used methods of light microscopy to study the role of microtubules in organelle transport and cell motility.  At Hopkins he taught courses in light and electron microscopy, and with the arrival of the school's first confocal microscope in 1988, helped establish the School of Medicine Microscope Facility, which he supervised until 2006.  He published the first edition of Fundamentals of Light Microscopy in 2001.  He is now retired but continues to teach light microscopy at Janelia Research Campus.

Michael W. Davidson - Mike was an Assistant Scholar/Scientist affiliated with the National High Magnetic Field Laboratory and the Department of Biological Science at the Florida State University.   Davidson’s laboratory created educational websites (including Molecular Expressions, Nikon MicroscopyU, Olympus Microscopy Resource Center, and Zeiss Campus), which continue to be valuable resources for light microscope optics and live cell imaging methods.  Davidson was also closely involved in research collaborations involving the performance of fluorescent proteins and optical highlighters used for labeling cellular structures and studying molecular dynamics in living cells.  Davidson’s digital images and photomicrographs have graced the covers of over 2000 publications in the past two decades and he has licensed images to numerous commercial partners.  Mike died at the end of 2015.

Interview with Doug Murphy

I&M: What was the reason to write the book?
Murphy: The purpose of the book was to lay out the basic theory and principles of light microscope optics, and describe principles of modern light microscopy, so that investigators could make optimal use of these instruments.  Most modern instruments integrate computers and software to control imaging processes electronically.  The immediate challenge to investigators is to learn how to operate the process of image acquisition, but often without instruction on the basic optical principles or essential adjustments that are necessary.  The book serves this need, by describing basic principles of image formation,  explaining the details of the different modes of microscopy, and reviewing the essentials of image processing and guidelines for image acquisition with the goal of scientific publication.

I&M: What are the problems every beginner in the field of Microscopy encounters?
Murphy: The main challenge is to appreciate that exposure to light is damaging to both chemical structure and biological function.   Therefore, one needs to learn how to adjust variables that affect exposure to light (exposure time, numerical aperture, electronic gain, laser scan rates, pinhole settings, and others).  Because the equipment affecting image quality and light exposure varies on different microscopes, the user must understand the optical principles and controls of each microscope system.  This responsibility extends even to post-acquisition image processing steps on the way to publication or display.

I&M: Will – in your opinion – the automated evaluation of images make the life of the scientist easier?
Murphy: Automated evaluation of dozens or hundreds of images (high content imaging) is essential for the modern laboratory.  However, the images evaluated are only valuable if certain measures have been applied to obtain and correct them.  It is the responsibility of the operator acquiring the images to use controls optimally, as described above.

More information:

Murphy, D. B. / Davidson, M. W.
Fundamentals of Light Microscopy and Electronic Imaging
Second edition
ISBN: 978-0-471-69214-0
Also available in digital formats.

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