Webinar: Integrated Cryo-Tomography Workflow
– Changing the way we look at the inner workings of cells
Understanding how cells, tissues and whole organisms work requires understanding how proteins and protein complexes function. Today the structure of individual proteins is determined by techniques such as X-ray crystallography, NMR and single-particle cryo-electron microscopy. However, analyzing purified protein structures alone only gives a partial picture of how they work, because the cellular context is missing.
The speakers will explain every sample preparation and imaging step as well as the unique features of the hard - and software integration.
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This webinar will also be available to view on demand.
- How the cryo-tomography workflow works in detail
- How cryo-light-microscopy fits into the workflow
- How cryo-electron tomography is used to image the cell interior at high resolution
- The latest technical developments for cryo-light microscopy
- EM and Cryo EM users
- Engineers and researchers who are interested in cryo preparation techniques
About the presenters:
Dr. Julia König studied Biology at Dresden University of Technology. For her Diploma and PhD thesis she joined the lab of Thomas Mueller-Reichert at the Max Planck Institute of Molecular Cell Biology and Genetics and the Medical Faculty at Dresden University of Technology. There she used correlative light and electron microscopy as well as electron tomography to analyse the ultrastructure of Nematode embryos during different stages of cell division.
After finishing her PhD in 2015, she joined the Electron Microscopy Facility of Lucy Collinson at the Francis Crick Institute in London, working on a wide variety of electron microscopy projects as well as methods and workflow development. In January 2018, she joined Leica Microsystems as a product manager for EM sample preparation for cryo workflows.
Dr. Alexander Rigort currently works as a product marketing manager for Thermo Fisher Scientific. He developed and used cryo-focused ion beam instrumentation for applications in electron tomography at the Max Planck Institute of Biochemistry in Munich and has more than 12 years’ experience in cryo-electron microscopy.