X-ray Diffraction Microscopy
H. Jiang and co-workers bridged the visualization gap between 3D optical microscopy and 3D electron microscopy by demonstrating the quantitative 3D imaging of a whole unstained cell by X-ray diffraction microscopy at a resolution of 50-60 nm. Subcellular structures and organelles could be identified and the resolution can be even further improved by cryotechnology.
Jiang H. et al.: Quantitative 3D imaging of whole, unstained cells by using X-ray diffraction microscopy. PNAS June 22, 2010 vol. 107 no. 25 p. 11234-11239 (2010)