Array Tomography


Dear Readers,

this special newsletter focuses on "Array Tomography", a method established by Stephen J. Smith at Stanford University in 2007.  

Array tomography is a 3D image reconstruction technique for biological specimens. It involves the imaging of ordered arrays of ultrathin serial sections with scanning electron microscopy (SEM) or confocal light microscopy and enables high resolution, quantitative analysis of biological structures. Array tomography offers higher spatial resolution than conventional confocal microscopy. The method can reveal additional information of cellular and protein structures to clarify poorly understood or unresolved features.

To obtain excellent results with array tomography in a timely manner, it is crucial to create ultrathin, consistent, and correctly ordered ribbons efficiently. Read more about this below.

Enjoy reading this newsletter.

Your Imaging & Microscopy team




Webinar: Array Tomography for SEM 3D Reconstruction


Array tomography (AT) is a 3D image reconstruction technique for high resolution, quantitative analysis of biological structures. For optimal results, ultrathin and ordered sections are an absolute requirement. Join this live webinar and ...


Fast, Precise Serial Sectioning for Array Tomography


Video showing the serial sectioning of mouse lymph nodes with the the ARTOS 3D and the generation of 3D images using array tomography. The video is also available here.




Faster 3D Imaging of Cellular and Protein Structures


An application note is now available which explains how you can better optimize your array tomography workflow using an automated serial sectioning solution, like the ARTOS 3D ultramicrotome from Leica Microsystems. Want to learn more ...


Answering the FAQs of Array Tomography

This method was first reported by Micheva and Smith in 2007. Recently a Q&A with Stephen J. ...


Creating Serial Ultrathin Sections for SEM Array Tomography

In a paper by Taro Koike et al., a new “Device for Ribbon Collection for Array Tomography ...


Correlative Microscopy Using SEM Array Tomography

Burel et al., recently presented an optimized scanning electron microscopy (SEM) workflow for ...




Holotomographic Microscopy: Label-Free 3D Analysis of Organelles


Mitochondria are one of the most important cell organelles. They are organized in extremely dynamic networks and, in addition to supplying the cell with energy, they have an important role in apoptosis, metabolism, and degenerative ...


Protein Visualization: New Method Refines Cell Sample Analysis

Innovation in the field of biomedicine: Researchers at the University of Zurich have developed a ...


Universal Pressure Scanning Electron Microscopy

Recent publications on high pressure capabilities of state of the art environmental scanning ...


Need for Speed

Chemical fixation followed by epon embedding and thin sectioning still is a powerful approach for ...




Simplify your Preparation for Array Tomography


The ARTOS 3D (ARray TOmography Solution) automatically creates and collects hundreds of serial-section ribbons all ready for array tomography with your scanning electron microscope (SEM). Save time and effort in biological sample ...

Imaging & Microscopy Issue 3/2019