Dynamic Live-Cell Processes in 3D Super Resolution with SpinSR by Olympus

  • IXplore Spin SR squareIXplore Spin SR square
  • IXplore Spin SR square
  • Super resolution images at different Z-positions of Purkinje cells labeled with GFP.
  • cellFRAP enables researchers to study cellular dynamics with high spatial and temporal resolution.

The Olympus IXplore SpinSR combines super resolution, confocal and widefield imaging in one easy-to-use system with fast switching between imaging modes. With the ability to resolve features down to 120 nm, IXplore SpinSR enables fast imaging of live cells with less phototoxicity and photobleaching. Olympus silicone immersion objectives improve brightness and reduce spherical aberration for accurate Z-axis information and better 3D reconstruction.

The combination of speed, resolution and efficiency make Olympus’ IXplore SpinSR ideal to cope with the challenges in live-cell imaging. Researchers can observe fine details and dynamics of cellular structures and processes with the ability to easily switch between super resolution, confocal, and widefield imaging.

With IXplore SpinSR, users can not only observe surface features, but also obtain 3D images of structures up to 100 µm below the surface. Olympus silicone immersion objectives are specially designed for this type of deep-tissue observation. The refractive index of silicone oil best matches that of living cells and cultured tissue slices, thereby reducing spherical aberration.

To harness the power of IXplore SpinSR for observing fast cellular processes, Olympus cellFRAP can be used for photomanipulation experiments. cellFRAP is equipped with a diffraction-limited laser for flexible, precise and easy photomanipulation. It can be easily adapted for IXplore SpinSR to capture fast in vivo processes with the highest spatial and temporal resolution. Its unrivalled short switchover time of only 200 µs between bleach and post-bleach acquisition ensures detection of all important sample responses immediately after stimulation.

Further information can be found here.

 

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Olympus Europa SE & Co. KG
Amsinckstraße 63
20097 Hamburg
Germany
Phone: +49 40 23773 0
Telefax: +49 40 23376 5

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