Zeiss LSM 800: A Compact Confocal Laser Scanning Microscope
- Comparison between confocal and Airyscan image. HeLa cells, red: mitochondria membrane, green: microtubuli, magenta: actin fibres; acquired with LSM 800. Scale bar 5 µm. Sample courtesy of A. Seitz, EPFL, Lausanne, Switzerland.
- Zeiss LSM 800 with Airyscan: Best imaging quality and fast linear scanning
Zeiss has introduced Zeiss LSM 800, a compact confocal laser scanning microscope for high-end confocal imaging. With highly sensitive GaAsP detector technology and fast linear scanning, the system provides high image quality and offers enhanced productivity and throughput, as well as greater flexibility in live cell imaging. The optional Airyscan module delivers 1.7 times higher resolution and higher sensitivity than conventional confocal microscopes.
Scientists can resolve even fast movements of labeled proteins thanks to the increased scanning speeds. At an image size of 512 x 512 pixels, they are able to capture events at up to 8 frames per second. Zeiss LSM 800 is continually monitoring and calibrating the scanner position to guarantee a stable field of view and equal pixel integration times over the whole field of view. The patented linear scanning regime gives a constant signal-to-noise level and uniform exposure to the illuminating laser throughout the scanned area, including manipulated regions of interest.
Gallium arsenide phosphide photomultiplier tubes (GaAsP PMTs) display high light collection efficiencies over a broad spectral range. Their low dark noise levels also make them the ideal tool for detecting faint signals in low expressing cells. The gain in signal-to-noise ratio (SNR) will increase productivity by achieving faster scan speeds while preserving high image quality. Low laser powers avoid photobleaching and phototoxicity in live cell imaging applications.