Imaging & Microscopy en Yimei Zhu Receives 2018 Distinguished Scientist Award from the Microscopy Society of America The Microscopy Society of America (MSA) has selected Yimei Zhu—a senior physicist and leader of the Electron Microscopy and Nanostructure Group in the Condensed Matter Physics and Materials Science Department at the U.S. Fri, 20 Apr 2018 12:21:00 +0000 Raman Microscopy: Cancer Drug Observed at Work Using a Raman microscope, researchers at Ruhr-Universität Bochum (RUB) have studied at which targets the cancer drug Neratinib binds in cells and how its chemical structure changes. Compared with other techniques, this method offers a considerable advantage, as it is not necessary to apply a label to the drug that would indicate its distribution indirectly; rather, the drug itself can be monitored. Fri, 20 Apr 2018 05:18:00 +0000 3D Imaging of Live Specimens The development of innovative tools for spatiotemporal 3D imaging of live, unlabeled biospecimens will significantly advance our understanding of problems ranging from cancer diseases and neuroscience to more accurate IVF procedures. Fri, 20 Apr 2018 04:18:00 +0000 Universal Pressure Scanning Electron Microscopy Recent publications on high pressure capabilities of state of the art environmental scanning electron microscopes have shown that they are working far away from physical limits and that there is plenty of room for improvements. In this publication an optimized pressure limiting aperture holder and secondary electron detection system is presented which pushes the limits. Imaging at higher chamber pressures up to one atmosphere investigations of living organisms and much more are possible. Introduction Wed, 18 Apr 2018 10:40:00 +0000 Webinar: Correlative Raman Imaging and SEM Correlative microscopy is a hybrid approach that looks at a sample with different microscope technologies, each optimized individually then later linked for a far more detailed analysis of chemical and structural features. A new correlative method is RISE – Raman Imaging and Scanning Electron – microscopy. With this technique, information on the chemical composition of a sample provided by the optical Raman microscope is correlated with structural features imaged by a high resolution scanning electron microscope. Tue, 17 Apr 2018 06:50:00 +0000 A High-Performance Microscope Dangling from Bungee Cords To produce images of individual atoms, a microscope shouldn't shake. A patented vibration damping system developed at the TU Wien enables images of the highest quality. Mon, 16 Apr 2018 05:27:00 +0000 Lifetime - A Proper Alternative „Way too complicated!“ - the frequently-heard complaint about fluorescence lifetime measurements. But this is set to change! Development of new technologies and new concepts for data evaluation mean that fluorescence lifetime imaging (FLIM) is about to become 10 times faster and as hassle-free as standard confocal imaging. Fluorescence Imaging Is More than You Think! Fri, 13 Apr 2018 08:30:00 +0000 Forbidden FRET Situations on Wafer-Thin Gold G protein-coupled receptors (GPCRs) are the major target molecules for drug discovery. Scientists at the University of Würzburg and the University Hospital Jena have now been able to explore the activation of these receptors in more detail by developing a modern microscopy technique. Increasing the Förster Resonance Energy Transfer (FRET) through the use of specially coated coverslips was the key. Thu, 12 Apr 2018 12:18:00 +0000 Livecyte Now Available Throughout USA and Canada Scientists in North America can now benefit from the high contrast, label-free, live cell imaging provided by Livecyte, following the appointment of Bulldog Bio as the Phasefocus distributor for USA and Canada. Thu, 05 Apr 2018 08:15:00 +0000 New TemCam-XF416 CMOS Camera from TVIPS The TemCam-XF416 is the latest development of TVIPS featuring an entirely new 4k×4k sensor design, which simultaneously satisfies different criteria. Large pixel size (16 µm x 16 µm), single electron sensitivity (high signal to noise ratio), fast acquisition rate, extended dynamic range, high spatial resolution and applicable for wide acceleration energy range (8 kV-400 kV). Thu, 05 Apr 2018 05:08:00 +0000 CMOS Sensor Technology at its Best from Basler Basler brings the Basler MED ace specifically designed for Medical & Life Sciences to market. The series offers powerful CMOS sensor technology, unique MED feature sets and compliance to DIN EN ISO 13485:2016 to address the market’s imaging challenges. Tue, 03 Apr 2018 13:59:00 +0000 Protochips New European Office Now Open Protochips, a company for in situ systems in electron microscopy, announced the official opening of its European office, Protochips EMEA GmbH. Tue, 27 Mar 2018 12:43:00 +0000 SCANDEM 2018 SCANDEM 2018 - The 69th Annual Conference of the Nordic Microscopy Society – will be held at the Technical University of Denmark Kgs. Lyngby in June 2018. Tue, 27 Mar 2018 06:03:00 +0000 X-Ray Imaging: Novel Lens Enables Microscopy with Nanometer Resolution A novel lens offers scientists the sharpest X-ray images yet from the nano world. The device is made from alternating layers of tungsten carbide and silicon carbide and can focus hard X-rays into a spot of less than ten nanometers in diameter as a team lead by Saša Bajt from the German research center Deutsches Elektronen-Synchrotron DESY report in Light: Science and Applications. Mon, 26 Mar 2018 12:29:00 +0000 Is Electron Counting Feasible with Indirect Camera Systems for TEM? Direct detection camera systems received a lot of praise in the last decade and are even referred to fuel a “Resolution Revolution” particularly in low-dose experiments [1]. This article compares the technological implications of direct and indirect detector designs and looks into the feasibility of implementing single electron (SE) counting acquisition schemes for a novel state of the art indirect CMOS camera system. Background Mon, 26 Mar 2018 06:45:00 +0000 Read & Win: Super-Resolution Microscopy - A Practical Guide Super-resolution microscopy is based on light microscopy and encompasses several techniques that enable an enhanced structural resolution in biological and other specimens down to 10 nm. It is the achievement of a truly interdisciplinary research community as it requires the expertise of chemists, biologists, physicists and engineers: Suitable probes such as chemical fluorophores and fluorescent proteins had to be designed, sample preparation optimized, and adequate hard- and software had to be developed for single-molecule detection and analysis. Mon, 26 Mar 2018 04:59:00 +0000 To See or Not to See.. In the years since their first appearance in 2010, scientific cameras based on scientific CMOS image sensors have had a large impact on numerous new technologies and methods in microscopy. Practically all camera based methods employing super resolution and nanoscopy have made use of the ideal combination, of low readout noise, high quantum efficiency and high frame rates associated with a very convenient pixel pitch of 6.5 µm, which fit perfectly to many microscope applications. sCMOS Image Sensors Are Improving Fri, 23 Mar 2018 09:00:00 +0000 Microsite on Failure Analysis and Quality Control in Material Sciences Leica Microsystems and Imaging & Microscopy have launched a Microsite to spotlight Leica’s` workflow solutions which cover all steps required for demanding high-quality sample preparation, whatever the nature of your sample. Learn from peer-reviewed scientific articles, how Leica Microsystems´ solutions cover all steps required for demanding high-quality sample preparation in various application areas. Fri, 23 Mar 2018 08:18:00 +0000 Atom Chains in the TEM The thinnest electrical conductor we can imagine is a linear chain of atoms. Carbon in the sp1-hybridization is able to form self-supporting and electrically conductive atom chains. These have been subject of theoretical studies for many years until it recently became possible to create and characterize individual carbon chains in in situ electron microscopy experiments. A combination of TEM and STM techniques sheds light on the properties of the ultimate nanowire. Atom Chains Fri, 23 Mar 2018 08:00:00 +0000 Femtosecond Stimulated Raman Microscopy Raman microscopy yields images with chemical contrast without relying on molecular labels. Its application is hampered by long acquisition times. This can be mitigated by applying non-linear Raman techniques. The one described here, Femtosecond Stimulated Raman Microscopy (FSRM), retains the spectral coverage of conventional Raman microscopy. Introduction Fri, 23 Mar 2018 07:00:00 +0000 Temperature Control for Live-Cell Imaging Thanks to microscopes, scientists start to observe smaller samples and are now able to observe atoms. In biology, samples might be alive but as a microscope stage is not their natural environment they tend to die after a short period of time. This limiting factor questions the relevance and robustness of the produced results as a results obtained from an organism/organ/cell cultured in a non physiological environment will always remain questionable. Thu, 22 Mar 2018 07:00:00 +0000 Cobolt Skyra: The New Multi-line Laser Cobolt AB proudly introduces the Cobolt Skyra, a revolutionary multi-line laser platform. Offering up to 4 laser lines, permanently aligned in a box that can fit into the palm of your hand. Wed, 21 Mar 2018 06:37:00 +0000 Measuring the Size Distribution of Nanomaterials FRAP is widely applicable in the biophysical, pharmaceutical and material sciences to study diffusion of molecules and nanoparticles on a micrometer scale. Here we extend the capability of FRAP to measuring polydisperse samples with a continuous distribution of diffusion coefficients (cFRAP). We demonstrate that cFRAP is a new analytical technique that allows to measure the size distribution of polydisperse nanomaterials, not only in buffer solutions but also in biological fluids. Introduction Fri, 16 Mar 2018 05:32:00 +0000 Leica EM TIC3X – Ion Beam Slope Cutter The Triple Ion Beam Milling System, Leica EM TIC 3X allows production of cross sections and planed surfaces for Scanning Electron Microscopy (SEM), Microstructure Analysis (EDS, WDS, Auger, EBSD) and, AFM investigations. Thu, 15 Mar 2018 10:21:00 +0000 Leica EM TXP – Target Surfacing System The Leica EM TXP is a target preparation device for milling, sawing, grinding, and polishing samples prior to examination by SEM, TEM, and LM techniques. An integrated stereomicroscope allows pinpointing and easy preparation of barely visible targets. Thu, 15 Mar 2018 10:16:00 +0000