Imaging & Microscopy en Ytterbium Fiber Lasers Enable Cutting-Edge Neuroscience Murine-based neuroscience research represents a major application segment for multiphoton microscopy. This article provides a glimpse into the field by examining a few interesting areas of investigation. As part of the quest toward understanding how cortical neuron networks operate well below the surface of brain tissue, red-shifted functional probes were developed; these are utilized in combination with deeper penetrating, long (i.e., > 1 micron) laser wavelengths. Fri, 14 Dec 2018 19:35:00 +0000 Ion Beam Preparation of Samples for SEM Download this 76-pages booklet today and learn how to improve your processes In this booklet you can find information about how ion beam milling can help you to: Fri, 14 Dec 2018 19:29:00 +0000 New Cryo-EM Center at Brookhaven Lab Today, the U.S. Department of Energy’s (DOE) Brookhaven National Laboratory broke ground on the Laboratory of BioMolecular Structure (LBMS), a state-of-the-art research center for life science imaging. At the heart of the center will be two new NY-State-funded cryo-electron microscopes (cryo-EM) specialized for studying biomaterials, such as complex protein structures. Fri, 14 Dec 2018 19:26:00 +0000 Oberserving Second-Harmonic Light EPFL researchers have developed a method to observe the electrical activity of neurons by analyzing the behavior of surrounding water molecules. This simple and non-invasive method, which could eliminate the need for electrodes and fluorophores, can be used to monitor the activity within a single neuron or potentially on an entire region of the brain. Wed, 12 Dec 2018 08:08:00 +0000 Boosting Resolution Scientists at the University of Würzburg have been able to boost current super-resolution microscopy by a novel tweak. They coated the glass cover slip as part of the sample carrier with tailor-made biocompatible nanosheets that create a "mirror effect". This method shows that localizing single emitters in front of a metal-dielectric coating leads to higher precision, brightness and contrast in Single Molecule Localization Microscopy (SMLM). Tue, 11 Dec 2018 06:43:00 +0000 COMULIS: Network of Correlative Multimodal Imaging COMULIS homepage is online now, the network of Correlative Multimodal Imaging (CMI) funded by the EU framework COST. You will find two open calls & more details on the homepage. This grant period will end already in April 2019. COMULIS will be funded till end of 2022. 1) STSM Call: Continuously Opened-Call, Applications for the STSMs can be submitted any time and will be reviewed at the end of each month! Mon, 10 Dec 2018 14:03:00 +0000 Content-aware Image Restoration Method lets Microscopists See More Modern microscopes can record many hours of 3D time-lapse movies of every cell as an organism develops. Just as for regular photography, fluorescence microscopy requires enough light to avoid dark and noisy images. However, the light necessary for such movies can easily reach levels that harm frequently studied model organisms such as worms, fish, and mice. To date, the only option to avoid this “ultimate sunburn” is to record shorter movies or reduce the amount of light used. Mon, 10 Dec 2018 10:10:00 +0000 Discover a New Level of Live-Cell Imaging Stable high-quality imaging at depth is vital for advanced life science applications. Olympus IXplore SpinSR confocal super-resolution microscope enables observation of dynamic changes and phenomena within live cells—thanks to a unique combination of speed, reduced phototoxicity, and stability. Mon, 10 Dec 2018 06:50:00 +0000 COBRA: X-Ray Visualization A team of materials scientists from Penn State, Cornell and Argonne National Laboratory have, for the first time, visualized the 3D atomic and electron density structure of the most complex perovskite crystal structure system decoded to date. Fri, 07 Dec 2018 13:13:00 +0000 Cryo-Electron Microscopy Shows How Stabilizing Proteins Bind to Microtubules By using three dimensional (3D) cryo-electron microscopy, RIKEN scientists have gleaned new insights into how stabilizing proteins bind to microtubules—the ‘conveyor belts’ of cells. These findings have implications for research into dementia and heart failure. Fri, 07 Dec 2018 12:45:00 +0000 New Series of Area Scan Camera Models and Software Integrations JAI has announced a series of new area scan camera models and software integrations which provide advanced color imaging capabilities to builders and users of microscopy-based imaging systems. Thu, 06 Dec 2018 17:23:00 +0000 On-Demand Webinar: New Technique for Quick 3D Imaging of Alloy Defects Aluminum (Al) alloys play an important role in the production of aircraft and vehicles, as well as products in other industries. Defects present in the Al alloy used for the production of aircraft, vehicles, or other products can have a significant effect on their quality, performance, and lifetime. Characterization of Al alloys in 3D can lead to a better understanding of defect formation and how to minimize or eliminate it. Wed, 05 Dec 2018 16:35:00 +0000 Atomic Jet Scientists from the Max Born Institute (MBI) have developed the first refractive lens that focuses extreme ultraviolet beams. Instead of using a glass lens, which is non-transparent in the extreme-ultraviolet region, the researchers have demonstrated a lens that is formed by a jet of atoms. The results, which provide novel opportunities for the imaging of biological samples on the shortest timescales, were published in Nature. Tue, 04 Dec 2018 11:45:00 +0000 First TIMA-X in China Operational at Geology Department of University of Peking Tescan is proud to announce that they have installed the first TIMA-X automated mineralogy system in China. The system was installed at the Geology Department at Peking University (PKU), Beijing.  PKU is ranked number 30 in the prestigious QS World University Rankings, with the Geology Department consistently being ranked as the top geological institution in the nation. Fri, 30 Nov 2018 08:36:00 +0000 Park Systems and University of Bologna are Proud to Announce the 2nd NanoScientific Forum Europe 2019 After the successful first edition of NanoScientific Forum Europe (NSFE) in October 2018 at the TU Freiberg, Germany Park Systems is happy to invite scientists and researchers working in the field of Atomic Force Microscopy to beautiful Bologna. The NSFE 2019 will be hosted by Dr. Tobias Cramer, Asst. Professor and Researcher at Department of Physics and Astronomy of University of Bologna, and co-organized by  CNR-ISMN Bologna, Italy. Fri, 30 Nov 2018 07:14:00 +0000 Gold Nanoparticle Microsecond Tracking with Atomic-level Localization Precision Optical microscopy enables us to observe motions of protein molecular motors as they work in the biological systems. By labeling molecules of interest with appropriate probes, the motions of individual molecules can be tracked. Localization precision (how precisely we can determine the position) of moving molecule is primary determined by the photon numbers captured by a single image frame. One nanometer localization precision and millisecond time resolution can be achieved with fluorescent probes, by capturing ~10,000 photons. Thu, 29 Nov 2018 08:14:00 +0000 Scanning Ion Conductance Microscopy – Scanning Electrochemical Microscopy Since the inception of scanning tunneling microscopy (STM) [1], electrochemists applied the scanning probe microscopy (SPM) technique to develop scanning electrochemical microscopy (SECM), also known as the chemical microscopy. Mon, 26 Nov 2018 13:40:00 +0000 Shaping the Electron Wavefronts The shape and intensity profile of the electron beam are critical in determining the final image quality that can be achieved in (scanning) transmission electron microscopy. Classic imaging theory would have us believe that optimal transmission electron microscopy images are formed with a wavefront that is as close to planar as possible, and that perfect scanning transmission electron microscopy images are made with probes formed from crisp circular apertures, with no aberrations. Mon, 26 Nov 2018 13:34:00 +0000 New Light Choice for Virtual Skin Biopsy Harmonic generation microscopy (HGM) is one of the most important techniques for optical virtual skin biopsy. The occurrence of optical harmonics mainly relies on the interaction between ultrafast laser pulses and the skin tissue. Conventionally, the burden to drive HGM falls on the shoulder of solid-state lasers. Here we demonstrate a novel fiber-based solution that combines ultrafast fiber laser technology and fiber-optic nonlinear wavelength conversion harnessing self-phase modulation (SPM). Mon, 26 Nov 2018 11:48:00 +0000 Multimodal Microscopy for Tissue Diagnostics Multimodal nonlinear microscopy using near infrared excitation lasers enables noninvasive, label-free high resolution imaging of native tissue. The methods are highly promising for biomedical applications, e.g., frozen section analysis in histopathology, in vivo intraoperative imaging and endoscopy, since disease induced morphologic and chemical changes can be directly visualized at subcellular spatial resolution without any need for contrast agents, when combined with novel laser technology. Mon, 26 Nov 2018 11:40:00 +0000 Well-Oiled Imaging In life science microscopy applications – such as 3D imaging of living specimens or long-term live-cell imaging – brightness, resolution and focus stability are key challenges. Silicone oil is an inert, stable immersion medium that helps to maintain focus in long-term imaging applications. It also has a refractive index close to living cells, which minimizes spherical aberrations in 3D – making silicone oil immersion objectives well suited for deep-tissue imaging of live cells. Mon, 26 Nov 2018 08:40:00 +0000 Zeiss Presents New Enhanced Software Technology Zeiss introduces new software modules with enhanced imaging technology: Zeiss ZEN Connect is especially useful for, but not limited to, structural analysis, examination of cellular processes, localization of cells, and much more. Thu, 22 Nov 2018 18:34:00 +0000 Cryo-EM Enables Visualization of Intraflagellar Transport Machines Inside a Cell Moving around, sensing the extracellular environment, and signaling to other cells are important for a cell to function properly. Responsible for those tasks are cilia, antenna-like structures protruding from most vertebrate cells. Whenever cilia fail to assemble correctly, their malfunctions can cause numerous human diseases. The assembly and maintenance of cilia requires a bidirectional transport machinery known as Intraflagellar Transport (IFT), which moves in train-like structures along the microtubular skeleton of the cilium. Wed, 21 Nov 2018 08:21:00 +0000 3D Chemical Maps of Single Bacteria Scientists at the National Synchrotron Light Source II (NSLS-II)—a U.S. Department of Energy (DOE) Office of Science User Facility at DOE’s Brookhaven National Laboratory—have used ultrabright x-rays to image single bacteria with higher spatial resolution than ever before. Tue, 20 Nov 2018 11:10:00 +0000 Making X-ray Microscopy 10 Times Faster Microscopes make the invisible visible. And compared to conventional light microscopes, transmission x-ray microscopes (TXM) can see into samples with much higher resolution, revealing extraordinary details. Researchers across a wide range of scientific fields use TXM to see the structural and chemical makeup of their samples—everything from biological cells to energy storage materials. Tue, 20 Nov 2018 10:30:00 +0000