Science Overview | Imaging & Microscopy - Research, Development, Production

Jul. 12, 2019
ScienceElectron and Ion Microscopy
Secondary Electron Energy Matters
Secondary Electron (SE) Imaging in Scanning Electron Microscopes is fundamental to Materials Science including polymers - yet it lacks nanoscale chemical information, unless samples are stained. Change comes when sets of images using SEs ...
Jul. 01, 2019
ScienceScanning Probe Microscopy
Determing the Elasticitiy of Graphene
Atomic force microscopy based nanoindentation technique provides a powerful approach to determine the elasticity of graphene. From the recorded force-displacement response, free standing graphene shows stiffness of 1TPa, which is the ...
Jun. 20, 2019
ScienceScanning Probe Microscopy
Stretching DNA Using Cryo-Force Spectroscopy
Mechanical properties of micrometer long DNA have mainly been probed at room temperature with a force resolution limited by thermal fluctuations. Cryo-force spectroscopy combined with computer simulations now enables to quantify adhesion ...
Jun. 19, 2019
ScienceImage Processing
The BigDataViewer Ecosystem
One of the defining characteristics of many types of modern microscopy is that we have well and truly entered the era of Big Data, thanks to ever-increasing spatial and temporal resolution. Once the size of a dataset gets beyond the local ...
Jun. 18, 2019
ScienceElectron and Ion Microscopy
ITO Substrates for CLEM of Fibril Proteins
Correlative light and electron microscopy in a SEM is usually performed using glass commercial substrates coated with indium-thin oxide. These substrates however show a patchy background unavoidably imaged when fibril proteins are ...
Apr. 15, 2019
ScienceElectron and Ion Microscopy
Electron Tomography of Whole Mounts
Rapid progress in the instrumentation, sample preparation methods, and computational power have triggered a revolution in electron tomography methods. Herein, we adapted a straightforward freeze-drying method using tert-butanol for ...
Apr. 12, 2019
ScienceLight Microscopy
Boosting Super-Resolution by Mirror-Enhanced dSTORM
Mirror-enhanced stochastic optical reconstruction microscopy (meSTORM) maximizes precision and signal-to-noise ratio in single molecule localization microscopy (SMLM): A coating on the microscopy glass cover slip creates a “mirror ...
Apr. 08, 2019
ScienceElectron and Ion Microscopy
Measuring the Bacterial Adhesion
To determine the antimicrobial efficacy, the research in the field of bioadhesion is crucial. Experiments include cultivations with E. coli K12 JM109 to reach bacteria adhesion in order to enable adhesion force measurements between ...

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