Compression and Crevasses in Vitreous Sections under Different Cutting Conditions

Wiley open access: A related research article, again with Jacques Dubochet in the authorship, reports on the compression and crevasses of vitreous sections under different conditions. In this work a Leica EM PACT was used to high-pressure freeze samples of Enterococcus feacalis. The cutting was performed using a Leica EM FC6 cryo-ultramicrotome.

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H.M. Han, B. Zuber; J. Dubochet; Compression and crevasses in vitreous sections under different cutting conditions; J Microsc. 2008 May;230(Pt 2):167-71. doi: 10.1111/j.1365-2818.2008.01972.x.

Compression and crevasses are common cutting artefacts in cryo-ultramicrotomy of vitreous sections. They can be reduced or suppressed under optimal cutting conditions. In the present study, compression and thickness were measured for different cutting speeds and knife angles. It was found that compression decreased with feed and that crevasses appeared only above a certain thickness. The optimal feed for vitreous sections was between 50 and 80 nm. The thickness, calculated by two independent methods, was quantitatively related to feed and compression.

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