Digital Holographic Cytometry
Macrophage Uptake of Nanoprobes
- Fig. 1: Schematic illustration of the HoloMonitor M4 and image reconstruction (PHIAB, Lund, Sweden).
- Fig. 2: RAW 264.7 cells were seeded at 6 x 104 cells per well and monitored for 48 h. A-C) SA-MIPs increased the average cell area, average cell thickness, and average cell volume, respectively. D) The number of cells was not affected by the SA-MIP treatment. All parameters were calculated using digital holographic cytometry. Error bars are based on the standard deviation.
- Fig. 3: Raw 264.7 macrophage cell were untreated (A) or treated with SA-MIPs (0.04 mg/ml) (B). Images of the cells were captured by digital holographic cytometer during 48 h. Framed area in the images show a disappearance of SA-MIPs after 48 h, due to an uptake by the cells (see video online). All colors are pseudocolors related to the thickness of the cells as shown by the color bar.
SA is a monosaccharide that plays an important role in the regulation of the innate immunity . The amount of SA has been found to correlate with cancer, with an upregulation on more aggressive cancers [13-15]. A SA-imprinted polymer shell was grafted from a silica core particle using deprotonated SA as template for MIP growth . Here we demonstrate how DHC can be used to analyze the possible uptake of SA-MIPs in macrophage cells in in vitro cultures.
The authors would like to thank the European Union’s Horizon 2020 research and innovation program under the Marie Sklodowska-Curie grant agreement No 721297 for financial support and Malmö University.
Time-lapse movie: macrophages ingesting nanoprobes: see video
Louise Sternbæk1,2, Martha Wamaitha Kimani3, Kornelia Gawlitza3, Birgit Janicke1, Kersti Alm1, and Anette Gjörloff Wingren2
1Phase Holographic Imaging AB, Lund, Sweden
2Department of Biomedical Sciences, Faculty of Health and Society, Malmö University, Malmö, Sweden
3Chemical and Optical Sensing Division, Bundesanstalt für Materialforschung und -prüfung (BAM), Berlin, Germany
Phase Holographic Imaging AB
Department of Biomedical Sciences
Faculty of Health and Society
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