Lensless Microscopy of Cell Cultures - Video to Figure 5, middle
Video to figure 5, middle: The segmented cells can be tracked over the whole experiment time to visualize cell migration, velocity, division rate and the cell lineage . These characteristics are used in chemotaxis assays, for example to analyze cell migration and division in wound healing assays . We visualized the tracks of several cells and noticed that, for instance mother cells, which cover a long distance between cell divisions hand down this attribute to their daughter cells.
The lensless Cell-Microscope combines holographic imaging of cells with a thermoelectric cooling system and automated cell segmentation for live cell imaging inside the incubator. Using holography instead of optical focusing enables to build a cost-efficient, robust and compact microscope. The cooling of the CMOS camera defines the local temperature of the integrated cell culture chamber. Thousands of cells can be imaged, segmented, counted and tracked continuously within the large field of view.