Lensless Microscopy of Cell Cultures - Video to Figure 5, right
Video to Figure 5, right: With the large cell number inside the field-of-view it is also more likely to observe rare events in the cell culture, such as tripolar cell division of mutated cells. The unusual division was easy to identify in the hologram and was reconstructed for detailed examination (fig. 5 right). Analyzing the degenerated cell in the timelapse video, it became clear, that the dividing cells could not separate during the cytokinesis and merge in one cell body again.
The lensless Cell-Microscope combines holographic imaging of cells with a thermoelectric cooling system and automated cell segmentation for live cell imaging inside the incubator. Using holography instead of optical focusing enables to build a cost-efficient, robust and compact microscope. The cooling of the CMOS camera defines the local temperature of the integrated cell culture chamber. Thousands of cells can be imaged, segmented, counted and tracked continuously within the large field of view.